Cytogenetic Characterization and AFLP-Based Genetic Linkage Mapping for the Butterfly Bicyclus anynana, Covering All 28 Karyotyped Chromosomes
2008

Genetic Mapping of the Butterfly Bicyclus anynana

Sample size: 92 publication 10 minutes Evidence: moderate

Author Information

Author(s): Van't Hof Arjen E., Marec FrantiĊĦek, Saccheri Ilik J., Brakefield Paul M., Zwaan Bas J.

Primary Institution: Leiden University

Hypothesis

What are the chromosome characteristics and genetic linkage in the butterfly Bicyclus anynana?

Conclusion

The study provides insights into the chromosome structure and evolution of Bicyclus anynana, along with a new method for linkage mapping.

Supporting Evidence

  • The study confirmed a haploid chromosome number of 28 for Bicyclus anynana.
  • A total of 347 AFLP markers and 9 microsatellites were used to cover all 28 chromosomes.
  • The study proposed a simpler and more reliable method of linkage mapping for Lepidoptera.

Takeaway

This study looks at the chromosomes of a butterfly and how they are linked to its traits, helping us understand how butterflies evolve.

Methodology

The study used cytogenetic analysis and AFLP-based genetic linkage mapping to examine the chromosomes of Bicyclus anynana.

Limitations

The W chromosome could not be mapped due to its lack of recombination.

Participant Demographics

The study involved a total of 71 males and 113 females from a full-sib cross design.

Digital Object Identifier (DOI)

10.1371/journal.pone.0003882

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