HIV-1 Subtype C Viruses and Monoclonal Antibodies
Author Information
Author(s): Gray Elin Solomonovna, Meyers Tammy, Gray Glenda, Montefiori David Charles, Morris Lynn
Primary Institution: National Institute for Communicable Diseases, Johannesburg, South Africa
Hypothesis
Are pediatric HIV-1 subtype C viruses sensitive to broadly neutralizing monoclonal antibodies raised against subtype B?
Conclusion
Only the antibody 4E10 showed significant activity against HIV-1 subtype C isolates, while 2G12 and 2F5 were ineffective.
Supporting Evidence
- None of the seven HIV-1 subtype C pseudovirions was sensitive to 2G12 or 2F5.
- Four viruses were sensitive to IgG1b12, and all seven viruses were sensitive to 4E10.
- The lack of crucial N-linked glycans correlated with the insensitivity to 2G12 and 2F5.
Takeaway
The study found that most HIV-1 subtype C viruses from children did not respond to certain antibodies that were expected to help prevent mother-to-child transmission.
Methodology
The study cloned envelope genes from HIV-1 subtype C viruses and tested their sensitivity to various monoclonal antibodies using a neutralization assay.
Limitations
The study used an artificial laboratory-based assay, which may not fully represent in vivo conditions.
Participant Demographics
Children with perinatally acquired HIV-1 infection from Johannesburg, South Africa.
Digital Object Identifier (DOI)
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