Gene Combination Transfer to Block Autoimmune Damage in Transplanted Islets of Langerhans
Author Information
Author(s): Suzanne Bertera, Angela M. Alexander, Megan L. Crawford, Glenn Papworth, Simon C. Watkins, Paul D. Robbins, Massimo Trucco
Primary Institution: University of Pittsburgh School of Medicine
Hypothesis
Can gene transfer to isolated donor islets protect them from autoimmune destruction without systemic immunosuppression?
Conclusion
The study shows that while gene combinations can be used to protect islet grafts from diabetogenic T cells, they do not significantly enhance graft function beyond that achieved with individual genes.
Supporting Evidence
- Gene transfer using adenoviral vectors was effective in delivering the transgenes to islet cells.
- Islet grafts expressing IRAP alone showed significantly longer function than controls.
- Combination of IRAP and MnSOD resulted in significantly longer graft function compared to controls.
Takeaway
Scientists are trying to help diabetic patients by using special genes to protect insulin-producing cells from being attacked by the immune system.
Methodology
The study involved gene transfer to isolated donor islets using adenoviral vectors, followed by transplantation into diabetic mice and evaluation of graft function after T-cell challenge.
Limitations
The study did not find a synergistic effect from combining genes, and the protective effects were not significantly greater than those of individual gene transfers.
Participant Demographics
NOD/LtSzJ female mice were used as islet donors and NOD.CB17-Prkdcscid/J female mice as recipients.
Statistical Information
P-Value
<.005
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website