Elemental analysis of lung tissue particles and intracellular iron content of alveolar macrophages in pulmonary alveolar proteinosis
2011

Analysis of Lung Particles in Pulmonary Alveolar Proteinosis

Sample size: 2 publication Evidence: moderate

Author Information

Author(s): Shimizu Yasuo, Matsuzaki Shinichi, Dobashi Kunio, Yanagitani Noriko, Satoh Takahiro, Koka Masashi, Yokoyama Akihito, Ohkubo Takeru, Ishii Yasuyuki, Kamiya Tomihiro, Mori Masatomo

Primary Institution: Gunma University Graduate School of Medicine

Hypothesis

The study aims to assess secondary pulmonary alveolar proteinosis (PAP) due to inhalation of harmful particles.

Conclusion

The study demonstrated that secondary PAP is associated with exposure to inhaled particles and accumulation of iron in alveolar macrophages.

Supporting Evidence

  • The study identified various elements in lung tissue from a PAP patient using in-air micro-PIXE analysis.
  • Iron accumulation was observed in alveolar macrophages from the PAP patient compared to normal lung tissue.
  • The major component of the inhaled particles was found to be silicon.

Takeaway

This study looked at lung tissue from two patients to see how inhaled particles and iron affect a rare lung disease called pulmonary alveolar proteinosis.

Methodology

In-air micro-PIXE analysis and Berlin blue staining were used to analyze lung tissue specimens.

Limitations

The study involved only two patients, which may limit the generalizability of the findings.

Participant Demographics

One patient was a 64-year-old woman who was a hairdresser and a current smoker; the other was a 72-year-old woman with lung cancer who was a never smoker.

Digital Object Identifier (DOI)

10.1186/1465-9921-12-88

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