Nop8p Controls Exosome during Ribosome Maturation
Author Information
Author(s): Santos Marcia C. T., Goldfeder Mauricio B., Zanchin Nilson I. T., Oliveira Carla C.
Primary Institution: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
Hypothesis
Nop8p may control the exosome function during pre-rRNA processing.
Conclusion
Nop8p inhibits the exosome activity, which is crucial for the maturation of ribosomal RNA.
Supporting Evidence
- Nop8p interacts with Nip7p and is required for 60S ribosome subunit biogenesis.
- Depletion of Nop8p leads to premature degradation of rRNAs.
- Nop8p inhibits the exosome complex activity in vitro.
- Nop8p mediates association of Nip7p to pre-ribosomal particles.
Takeaway
Nop8p is a protein that helps make ribosomes by controlling another complex called the exosome, which breaks down RNA. If Nop8p is missing, the exosome can destroy important RNA needed for ribosomes.
Methodology
The study involved cloning deletion mutants of Nop8p, analyzing rRNA levels through northern blot and qPCR, and performing in vitro assays to test the interaction between Nop8p and the exosome.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website