TP53 and MDM2 Gene Polymorphisms and Hepatocellular Carcinoma Risk in Italy
Author Information
Author(s): Di Vuolo Valeria, Buonaguro Luigi, Izzo Francesco, Losito Simona, Botti Gerardo, Buonaguro Franco M, Tornesello Maria Lina
Primary Institution: National Cancer Institute "Fond. Pascale", Naples, Italy
Hypothesis
The study investigates the association of TP53 codon 72 and MDM2 SNP309 polymorphisms with the risk of developing hepatocellular carcinoma among Italian patients.
Conclusion
The MDM2 SNP309 G allele is significantly associated with an increased risk of developing viral hepatitis-related hepatocellular carcinoma in the Italian population.
Supporting Evidence
- MDM2 SNP309 G/G and T/G genotypes were significantly more prevalent in HCC cases compared to controls.
- The study included 61 cases of hepatocellular carcinoma and 122 healthy controls.
- Frequencies of TP53 codon 72 alleles were not significantly different between cases and controls.
- MDM2 SNP309 G allele was associated with a higher risk of hepatocellular carcinoma.
- Statistical analyses showed significant differences in genotype frequencies for MDM2 SNP309.
Takeaway
This study found that a specific gene variant (MDM2 SNP309 G allele) can increase the risk of liver cancer in people from Italy, while another variant (TP53 codon 72) does not seem to affect this risk.
Methodology
The study analyzed the genotype distribution of TP53 codon 72 and MDM2 SNP309 in 61 hepatocellular carcinoma cases and 122 healthy controls using PCR and RFLP.
Potential Biases
The age and sex differences between cases and controls may introduce bias, although Hardy-Weinberg equilibrium was fulfilled.
Limitations
The study had a modest sample size and cases were not well matched for age and sex with the control subjects.
Participant Demographics
The study included 61 hepatocellular carcinoma cases (48 males, 13 females) and 122 healthy controls (all males).
Statistical Information
P-Value
0.006 for MDM2 SNP309 G/G genotype
Confidence Interval
95% CI = 1.3-9.7 for MDM2 SNP309 G/G genotype
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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