Complete Genome Sequence of Mycoplasma suis and Insights into Its Biology and Adaptation to an Erythrocyte Niche
Author Information
Author(s): Guimaraes Ana M. S., Santos Andrea P., SanMiguel Phillip, Walter Thomas, Timenetsky Jorge, Messick Joanne B.
Primary Institution: Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana, United States of America
Hypothesis
The objective was to use whole genome sequencing and analysis of M. suis to define pathogenicity mechanisms and biochemical pathways.
Conclusion
Genome analysis shows that M. suis is dependent on host cell metabolism, which is likely linked to its pathogenicity.
Supporting Evidence
- The M. suis genome consists of a single, 742,431 bp chromosome with low G+C content of 31.1%.
- A total of 844 coding DNA sequences (CDS) were predicted, with only 34.7% having a known function.
- M. suis is a glycolytic species, obtaining energy through sugars fermentation and ATP-synthase.
- The absence of key metabolic pathways suggests M. suis relies heavily on nutrients from its host.
- Genome analysis indicates that M. suis may cause disease by scavenging and competing for host nutrients.
Takeaway
Scientists studied a tiny germ called Mycoplasma suis that makes pigs sick by living in their blood. They found out how it survives and what it needs to grow.
Methodology
Whole genome sequencing was performed using GS-FLX (454) and Titanium chemistry, followed by data analysis and annotation.
Limitations
The study does not provide experimental evidence for all predicted metabolic pathways and functions.
Participant Demographics
One 45-day old, female, mixed-breed pig was used in the study.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website