A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus
2011

Multiple Antifungals Support Leafcutter Ants

publication Evidence: moderate

Author Information

Author(s): Seipke Ryan F., Barke Jörg, Brearley Charles, Hill Lionel, Yu Douglas W., Goss Rebecca J. M., Hutchings Matthew I.

Primary Institution: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom

Hypothesis

We hypothesize that production of multiple antifungals with different targets by single Actinobacterial species would make them more attractive to the ants as mutualists.

Conclusion

The study concludes that a single Streptomyces strain produces multiple antifungal compounds that help protect leafcutter ants' fungus gardens from pathogens.

Supporting Evidence

  • Streptomyces S4 produces candicidin and multiple antimycin compounds.
  • Disruption of the candicidin and antimycin gene clusters did not affect bioactivity against the pathogen Escovopsis.
  • The genome analysis identified multiple antibiotic biosynthetic pathways in the Streptomyces strain.

Takeaway

This study found that a type of bacteria helps ants by making different medicines to keep their fungus healthy and safe from bad germs.

Methodology

Genome sequencing and analysis were performed to identify antibiotic biosynthetic pathways, followed by gene disruptions and mass spectrometry to assess antifungal activity.

Limitations

The study does not identify all antifungal compounds produced by the Streptomyces strain, leaving some questions about its full biosynthetic capabilities.

Digital Object Identifier (DOI)

10.1371/journal.pone.0022028

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