Defining the Nuclear Proteome
Author Information
Author(s): Fink J Lynn, Karunaratne Seetha, Mittal Amit, Gardiner Donald M, Hamilton Nicholas, Mahony Donna, Kai Chikatoshi, Suzuki Harukazu, Hayashizaki Yosihide, Teasdale Rohan D
Primary Institution: ARC Centre of Excellence in Bioinformatics, The University of Queensland
Hypothesis
What is the composition of the nuclear proteome in mammals?
Conclusion
The study provides direct evidence that the nuclear proteome consists of at least 14% of the entire mammalian proteome.
Supporting Evidence
- Direct evidence for 2,568 mammalian proteins within the nuclear proteome was reported.
- The dataset represents at least 14% of the entire mammalian proteome.
- 1,529 proteins were localized using a high-throughput subcellular localization protocol.
- An additional 1,039 proteins had clear experimental evidence documented in literature.
Takeaway
Scientists found a lot of proteins in the nucleus of cells, which helps us understand how cells work.
Methodology
High-throughput localization assays were used to identify nuclear proteins in mouse cells.
Potential Biases
The initial dataset was biased towards transcription factors.
Limitations
The study may not account for tissue-specific variations in protein localization.
Digital Object Identifier (DOI)
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