Type 2 Diabetes and Inflammation Response in Muscle
Author Information
Author(s): Andreasen Anne Sofie, Kelly Meghan, Berg Ronan Martin Griffin, Møller Kirsten, Pedersen Bente Klarlund
Primary Institution: University Hospital Rigshospitalet, Copenhagen, Denmark
Hypothesis
The inflammatory stimulus, an intravenous bolus injection of Escherichia coli LPS, would increase the activity of intermediates associated with insulin resistance more profoundly in patients with type 2 diabetes.
Conclusion
Patients with type 2 diabetes exhibit a different inflammatory response in skeletal muscle compared to those with normal glucose tolerance, which may contribute to insulin resistance.
Supporting Evidence
- Patients with type 2 diabetes showed increased NF-κB binding activity after LPS.
- JNK phosphorylation was more pronounced in diabetic patients compared to those with normal glucose tolerance.
- AMPK phosphorylation increased in volunteers with normal glucose tolerance but not in those with diabetes.
Takeaway
This study looked at how inflammation affects muscle in people with type 2 diabetes. It found that their muscles react differently to inflammation than those of people without diabetes.
Methodology
The study involved 10 patients with type 2 diabetes and 10 participants with normal glucose tolerance who received an intravenous bolus of E. coli lipopolysaccharide (LPS) and had muscle biopsies taken at various time points.
Potential Biases
Potential variability in human responses and small sample sizes may introduce bias.
Limitations
The study had a small sample size and did not investigate other important signaling pathways that may affect insulin sensitivity.
Participant Demographics
10 patients with type 2 diabetes and 10 age-matched participants with normal glucose tolerance.
Statistical Information
P-Value
p<0.05
Confidence Interval
95% CI
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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