Impact of Anesthesia on Hemoglobin Levels in Mice
Author Information
Author(s): Anita Gothelf, Pernille Hojman, Julie Gehl
Primary Institution: Copenhagen University Hospital Herlev
Hypothesis
Does anesthesia with fentanyl/fluniasone and midazolam influence hemoglobin measurements in mice?
Conclusion
Anesthesia significantly decreases hemoglobin levels in mice, which should be considered in studies measuring hemoglobin.
Supporting Evidence
- Anesthesia with fentanyl/fluniasone and midazolam significantly decreased hemoglobin levels in mice.
- The mean hemoglobin level dropped from 9.9 mmol/L to 8.7 mmol/L after anesthesia.
- The decrease in hemoglobin levels lasted for more than 30 minutes after anesthesia.
Takeaway
When mice are put to sleep for experiments, their blood can show lower hemoglobin levels, which can confuse the results.
Methodology
A cross-over study comparing hemoglobin levels in anesthetized versus non-anesthetized mice.
Potential Biases
Potential bias from the effects of anesthesia on hemoglobin levels.
Limitations
The study may be affected by the physiological changes due to repeated blood sampling.
Participant Demographics
Female NMRI mice, 15 weeks old.
Statistical Information
P-Value
p < 0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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