The Chop Gene Contains an Element for the Positive Regulation of the Mitochondrial Unfolded Protein Response Regulation of CHOP by mtUPR
2007
The Chop Gene and Mitochondrial Stress Response
publication
Evidence: moderate
Author Information
Author(s): Tomohisa Horibe, Nicholas J. Hoogenraad
Primary Institution: La Trobe University
Hypothesis
The transcriptional activation of the chop gene is regulated by the mitochondrial unfolded protein response (mtUPR).
Conclusion
The study identifies an mtUPR response element in the chop promoter and suggests that CHOP can be induced by different stress response pathways.
Supporting Evidence
- The study shows that CHOP is activated by the accumulation of unfolded proteins in mitochondria.
- Deletion analysis revealed that the transcriptional activation of the chop gene is through an AP-1 element.
- The research indicates that CHOP can be induced by both mitochondrial and endoplasmic reticulum stress responses.
Takeaway
When proteins in mitochondria get messed up, a special gene called chop helps fix them, and this study shows how that gene gets turned on.
Methodology
The study involved deletion analysis of the chop promoter and assays using luciferase and GFP reporter constructs in COS-7 cells.
Digital Object Identifier (DOI)
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