Dietary Linoleic Acid and Tissue Arachidonic Acid in Mice
Author Information
Author(s): Weldon Kylie A, Whelan Jay
Primary Institution: University of Tennessee, Knoxville
Hypothesis
Dietary linoleic acid (LA) promotes chronic and acute diseases in humans by enriching tissues with arachidonic acid (AA).
Conclusion
Interspecies comparisons for dietary LA between rodents and humans can be achieved when rodents are provided human equivalent doses based on differences in metabolic activity.
Supporting Evidence
- Increasing dietary linoleic acid had little effect on tissue arachidonic acid levels.
- Supplementing diets with arachidonic acid significantly increased tissue arachidonic acid levels.
- The study provides a model for translating dietary effects from rodents to humans.
Takeaway
This study looked at how eating linoleic acid affects the levels of another fat, arachidonic acid, in mice, and found that giving mice the same amount of linoleic acid that humans eat doesn't change their arachidonic acid levels much.
Methodology
C57BL/6J mice were divided into 9 groups and fed diets with varying levels of linoleic acid and arachidonic acid, with changes in fatty acid compositions monitored in plasma and erythrocytes.
Limitations
The results cannot be extrapolated to all tissues.
Participant Demographics
C57BL/6J male mice, 6-7 weeks of age.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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