Cellular Signatures in CHO Cells for Antibody Production
Author Information
Author(s): Bai Ying, Mercadé Ivan Domenech, Elgendy Ramy, Lambiase Giulia, Peak-Chew Sew, Franco Catarina, Wingett Steven W., Stevens Tim J., Grassi Luigi, Hitchcock Noah, Ferreira Cristina Sayago, Hatton Diane, Miller Elizabeth A., Mistry Rajesh K.
Primary Institution: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
Hypothesis
How do biological pathways change with antibody titre in CHO cells?
Conclusion
The study identifies that transgene copy number and mRNA expression levels are key drivers of antibody production in CHO cells.
Supporting Evidence
- Transgene copy number and mRNA expression levels correlate with antibody production.
- High and low antibody secreting clones were identified for each biotherapeutic.
- Multi-omics analysis revealed limited signatures associated with productivity.
Takeaway
This study looks at how certain cells can make more antibodies and finds that having more copies of the antibody gene helps them do this better.
Methodology
The study used quantitative transcriptomic and proteomic analyses to investigate gene and protein expression profiles in CHO cells during antibody production.
Limitations
The heterogeneity of clonal cell lines may mask the ability to detect cellular adaptations.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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