Syndecan-1 mediates internalization of apoE-VLDL through a low density lipoprotein receptor-related protein (LRP)-independent, non-clathrin-mediated pathway
2006
Syndecan-1 and ApoE-VLDL Uptake in Human Fibroblasts
publication
Evidence: high
Author Information
Author(s): Wilsie Larissa C, Gonzales Amanda M, Orlando Robert A
Primary Institution: University of New Mexico, School of Medicine
Hypothesis
Syndecan-1 mediates the internalization of apoE-VLDL through a pathway independent of LRP.
Conclusion
Syndecan-1 can mediate the uptake of apoE-VLDL in human fibroblasts without relying on LRP.
Supporting Evidence
- Syndecan-1 expression restored apoE-VLDL binding and uptake in GM00701 cells.
- Uptake of apoE-VLDL was unaffected by LRP antagonists but inhibited by heparin.
- Syn-1 internalization of apoE-VLDL was shown to be clathrin-independent.
Takeaway
Syndecan-1 helps cells take in a type of fat-carrying particle called apoE-VLDL, even when another receptor, LRP, isn't working.
Methodology
The study used a human fibroblast cell line (GM00701) to investigate the role of syndecan-1 in the uptake of apoE-VLDL.
Digital Object Identifier (DOI)
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