Group II Phospholipase A in Human Gingiva with Periodontal Disease
Author Information
Author(s): H. Shinohara, T. Komatsubara, H. Tojo, M. Okamoto, S. Nishikawa, T. Nagata, H. Ishida
Primary Institution: Department of Periodontology and Endodontology, School of Dentistry, the University of Tokushima; Department of Molecular Physiological Chemistry, Osaka University Medical School
Hypothesis
The study aims to determine the type and level of phospholipase A in gingival tissue from patients with periodontal disease.
Conclusion
The phospholipase A activity found in the gingival crevicular fluid of patients with periodontal disease is attributed to the II-PLA group, not the I-PLA group.
Supporting Evidence
- II-PLA levels increased with gingival tissue weight in a dose-dependent manner.
- No I-PLA was found in any of the samples tested.
- A significant correlation was observed between the level and activity of II-PLA2.
- Inflammatory cytokines induced II-PLA2 synthesis in gingival cells.
Takeaway
This study found that a specific enzyme related to inflammation is present in the gums of people with gum disease, which can help doctors understand how active the disease is.
Methodology
Gingival tissues were collected from patients and analyzed using biochemical and radioimmunological methods to measure phospholipase A activity and levels.
Limitations
The study could not identify the specific source of II-PLA2 in gingival tissue.
Participant Demographics
Patients had a mean age of 42 years and had at least five teeth with a pocket depth of more than 6 mm.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
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