Impact of device variability and protocol differences on kidney function during normothermic machine perfusion: A comparative study using porcine and human kidneys
2025

Impact of Device Variability on Kidney Function During Machine Perfusion

Sample size: 12 publication 10 minutes Evidence: moderate

Author Information

Author(s): Lantinga Veerle A., Arykbaeva Asel S., Spraakman Nora A., Blom Elwin W. P., Huijink Tobias M., de Vries Dorottya K., Ploeg Rutger J., Alwayn Ian P. J., Leuvenink Henri G. D., Moers Cyril, van Leeuwen L. Leonie

Primary Institution: University Medical Center Groningen

Hypothesis

Different perfusion protocols and devices will significantly affect kidney function and perfusion parameters during normothermic machine perfusion.

Conclusion

The study found that perfusion characteristics and kidney function are significantly influenced by the perfusion protocol and the device used during normothermic machine perfusion.

Supporting Evidence

  • The PL group had significantly lower arterial pO2 compared to the KA groups.
  • Oxygen consumption was significantly higher in the PL group compared to the KA groups.
  • The modified KA group showed significantly lower vascular resistance and higher oxygen delivery in human kidneys.

Takeaway

This study shows that how you pump blood into a kidney can change how well it works, so it's important to use the right method.

Methodology

Porcine kidneys were perfused using different devices and protocols, and human kidneys were tested with the best protocol identified.

Potential Biases

The study may have biases due to the small sample size and the nature of the experimental design.

Limitations

The sample size is small, limiting generalizability, and the kidneys were not transplanted, so the impact on transplant outcomes is unknown.

Participant Demographics

Porcine kidneys from six-month-old Dutch Landrace pigs and discarded human kidneys.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1111/aor.14851

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