The Testis-Specific Factor CTCFL Cooperates with the Protein Methyltransferase PRMT7 in H19 Imprinting Control Region Methylation
2006
New Factors Controlling Parent-Specific Genetic Control
publication
Evidence: moderate
Author Information
Author(s): Petar Jelinic, Jean-Christophe Stehle, Phillip Shaw
Hypothesis
The study investigates the role of the testis-specific factor CTCFL in the methylation of the imprinting control region (ICR) associated with the Igf2/H19 genes.
Conclusion
The study identifies CTCFL and PRMT7 as key factors in the paternal methylation of the ICR, influencing gene regulation.
Supporting Evidence
- CTCFL binds to the imprinting control region and recruits enzymes that direct methylation.
- CTCFL significantly stimulates the efficiency of PRMT7 in methylating histone proteins.
- Injection of CTCFL, PRMT7, and Dnmt3 into frog eggs resulted in significant DNA methylation.
Takeaway
This study found that a special protein helps control how genes from our parents are turned on or off, especially in the case of the Igf2 and H19 genes.
Methodology
The researchers used genetic assays and injections into frog eggs to test the interactions and effects of CTCFL, PRMT7, and Dnmt3 on DNA methylation.
Digital Object Identifier (DOI)
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