Regulation of Translation in Haloarchaea: 5′- and 3′-UTRs Are Essential and Have to Functionally Interact In Vivo
2009
Regulation of Translation in Haloarchaea
publication
Evidence: high
Author Information
Author(s): Brenneis Mariam, Soppa Jörg
Primary Institution: Goethe-University, Institute for Molecular Biosciences, Frankfurt, Germany
Hypothesis
The study investigates the role of untranslated regions (UTRs) in differential translational control in haloarchaea.
Conclusion
Both 5′- and 3′-UTRs are essential and sufficient for differential translational regulation in haloarchaea.
Supporting Evidence
- The study provides the first examples that both 5′- and 3′-UTRs are essential for translational regulation in a prokaryote.
- Translational regulation was completely abolished when stem loops in the 5′-UTR were changed by mutagenesis.
- The direction of translational regulation is encoded in the 3′-UTR, not in the 5′-UTR.
Takeaway
This study shows that parts of the gene that don't code for proteins help control how much of the protein is made, which is important for the cell's response to different conditions.
Methodology
The study used a reporter gene system to analyze translational efficiencies and the effects of UTRs on RNA stability and translational regulation.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website