The pH partition theory predicts the accumulation and toxicity of doxorubicin in normal and low-pH-adapted cells
1999
Doxorubicin and pH Levels in Cells
publication
Author Information
Author(s): Gerweck L E, Kozin S V, Stocks S J
Primary Institution: Department of Radiation Oncology, Massachusetts General Hospital, Boston, MA, 02114, USA
Hypothesis
How does extracellular pH affect the accumulation and toxicity of doxorubicin in normal and low-pH-adapted cells?
Conclusion
Doxorubicin accumulation and toxicity increase with higher extracellular pH, but normal cells show more toxicity than low-pH-adapted cells at the same pH.
Supporting Evidence
- Low-pH-adapted cells have different pH gradients compared to normal cells.
- Doxorubicin's toxicity is more pronounced in normal cells at the same extracellular pH.
Takeaway
This study found that the acidity of the environment affects how much of a cancer drug, doxorubicin, can get into cells and how harmful it is to them.
Methodology
The study investigated doxorubicin's behavior in cells cultured under different pH conditions.
Digital Object Identifier (DOI)
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