HIV-1 and its Effects on p53 in CD4+ T Cells
Author Information
Author(s): Imbeault Michaël, Ouellet Michel, Tremblay Michel J
Primary Institution: Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval, and Faculté de Médecine, Université Laval, Québec, Canada
Hypothesis
HIV-1 induces changes in gene expression in human primary CD4+ T lymphocytes.
Conclusion
HIV-1 infection leads to the up-regulation of p53 mRNA in CD4+ T cells, which is associated with type-I interferon production.
Supporting Evidence
- HIV-1 significantly influenced the transcriptomic profile of CD4+ T cells.
- 404 genes were modulated at least twofold by HIV-1.
- p53 mRNA was up-regulated by HIV-1 at both 8 and 24 hours post-infection.
- Type-I interferon production was linked to the up-regulation of p53 mRNA.
- Low infection rates were observed in primary human CD4+ T cells compared to cell lines.
Takeaway
When HIV-1 infects certain immune cells, it makes them produce more of a protein called p53, which helps control cell death.
Methodology
The study used microarray technology to analyze gene expression changes in CD4+ T cells after HIV-1 exposure.
Potential Biases
Potential contamination of CD4+ T cells with other cell types could influence results.
Limitations
The study's findings may not fully represent the in vivo situation due to the low infection rates in primary cells.
Participant Demographics
Healthy donors were used to isolate CD4+ T cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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