Dengue Virus Type-3 Outbreak in India
Author Information
Author(s): Dash Paban Kumar, Parida Man Mohan, Saxena Parag, Abhyankar Ajay, Singh CP, Tewari KN, Jana Asha Mukul, Sekhar K, Rao PV Lakshmana
Primary Institution: Division of Virology, Defence R&D Establishment, Gwalior, India
Hypothesis
The study investigates the reemergence of dengue virus type-3 (subtype III) in India and its implications for increased incidence of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).
Conclusion
The study confirms that the major dengue outbreak in northern India in 2003 and 2004 was caused by dengue virus type-3 (subtype III), which has replaced the previously circulating subtype IV.
Supporting Evidence
- The serological investigation revealed 11% IgM, 51% IgG, and 38% both IgM and IgG positivity.
- RT-PCR analysis confirmed the presence of dengue RNA in 17 samples.
- Subtyping revealed the dominance of subtype III of dengue virus type 3 in the outbreak.
Takeaway
This study shows that a new type of dengue virus is making people sick in India, and it's important to keep an eye on it to help prevent more serious illnesses.
Methodology
The study involved serological, virological, and molecular investigations of dengue outbreaks using RT-PCR, nested PCR, and virus isolation.
Limitations
The study may have limitations in sample collection timing, as many patients presented after the viraemic phase, potentially affecting RT-PCR positivity.
Participant Demographics
The majority of patients were over 25 years old, with a male to female ratio of 2.6:1.
Digital Object Identifier (DOI)
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