Predicting Protein Structures Using Functional Sites
Author Information
Author(s): Chelliah Vijayalakshmi, Taylor William R
Primary Institution: Division of Mathematical Biology, The National Institute for Medical Research
Hypothesis
Can predicted functional site positions help in selecting correct protein models from decoy structures?
Conclusion
The method effectively discriminates native-like protein models from non-native ones using predicted site positions.
Supporting Evidence
- The method was tested on five small α/β/α proteins.
- It ranked models based on the proximity of predicted functional residues.
- The method was applied to larger proteins with successful identification of correct folds.
Takeaway
Scientists can use clues about where proteins do their jobs to figure out which shapes are the right ones for those proteins.
Methodology
The study used a scoring method based on the proximity of predicted functional site residues to rank protein models.
Limitations
The method may not accurately predict models with swapped strands or loops that do not correspond to functional sites.
Digital Object Identifier (DOI)
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