Detecting Blood MicroRNAs Linked to Cancer in Mice
Author Information
Author(s): Fan Alice C, Goldrick Marianna M, Ho Jennifer, Liang Yu, Bachireddy Pavan, Felsher Dean W
Primary Institution: Stanford University, School of Medicine
Hypothesis
Can microRNAs associated with malignancy be detected in the peripheral blood of transgenic mice?
Conclusion
Specific changes in blood microRNA can be detected during tumorigenesis and tumor regression.
Supporting Evidence
- Distinct miRNA profiles have been described for many cancers.
- High concordance was observed in relative levels of blood miRNAs.
- Changes in specific miRNAs can be detected in the blood of transgenic mice with lymphoma, hepatocellular carcinoma, and osteosarcoma.
Takeaway
The study shows that we can find special tiny molecules in the blood of mice that can tell us if they have cancer or if their cancer is getting better.
Methodology
Real-time reverse transcriptase-PCR was used to analyze miRNA profiles in whole blood from transgenic mice with various cancers.
Limitations
The source of the detected miRNA changes could not be determined.
Participant Demographics
Transgenic mice with c-MYC-induced lymphoma, hepatocellular carcinoma, and osteosarcoma.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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