AP-1 Is a Component of the Transcriptional Network Regulated by GSK-3 in Quiescent Cells
2011
GSK-3 and AP-1 in Quiescent Cells
publication
Evidence: moderate
Author Information
Author(s): Tullai John W., Tacheva Silvia, Owens Laura J., Graham Julie R., Cooper Geoffrey M.
Primary Institution: Boston University
Hypothesis
GSK-3 regulates the transcriptional network involving AP-1 in quiescent cells.
Conclusion
Inhibition of c-Jun by GSK-3 contributes to the repression of growth factor-inducible genes in quiescent cells.
Supporting Evidence
- GSK-3 is active in quiescent cells and regulates gene expression.
- c-Jun is phosphorylated by GSK-3, which inhibits its activity.
- Inhibition of GSK-3 leads to increased binding of c-Jun to target genes.
Takeaway
GSK-3 helps keep certain genes quiet when cells are not growing, and it does this by controlling a protein called c-Jun.
Methodology
Chromatin immunoprecipitation analysis was used to study the binding of c-Jun and JunD to GSK-3-regulated genes.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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