Host Kinases Involved in Listeria Spread
Author Information
Author(s): Chong Ryan Squires, Raynal Swiss, Rachel Agaisse, Hervé Agaisse
Primary Institution: Yale University School of Medicine
Hypothesis
What host factors are required for the formation and resolution of membrane protrusions during Listeria monocytogenes spread from cell to cell?
Conclusion
The study identifies CSNK1A1 and CSNK2B as specific host kinases required for Listeria monocytogenes to form productive membrane protrusions and spread from cell to cell.
Supporting Evidence
- CSNK1A1 and CSNK2B were identified as key kinases for Listeria spread.
- CSNK1A1 is required in sending cells for protrusion resolution.
- CSNK2B is involved in cytosolic actin-based motility.
Takeaway
This study found that certain proteins in our cells help the Listeria bacteria move from one cell to another, which is important for how they spread.
Methodology
The researchers used a high-throughput RNAi screen targeting the human kinome to identify kinases involved in Listeria spread, followed by validation of candidate genes.
Potential Biases
The RNAi methodology may have unintended silencing of genes with limited sequence homology.
Limitations
The study faced high rates of off-target effects in RNAi screening, leading to potential false positives.
Participant Demographics
Human epithelial cells were used for the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website