Leukocyte Behavior and Vascular Leakage in Sepsis
Author Information
Author(s): Steven M Hollenberg, Massimiliano Guglielmi, Joseph E Parrillo
Primary Institution: Cooper University Hospital
Hypothesis
Abrogation of inducible nitric oxide synthase (iNOS) activation would decrease leukocyte rolling, leukocyte adhesion, and microvascular leakage in sepsis.
Conclusion
Leukocyte adhesion and vascular leakage were discordant in this setting, indicating an important role for nitric oxide in modulating vascular permeability during sepsis.
Supporting Evidence
- Leukocyte rolling and adhesion were significantly increased in septic animals compared to controls.
- Microvascular leakage was significantly lower in iNOS-deficient septic mice compared to wild-type septic mice.
- The study suggests that increased vascular permeability in sepsis is dependent on iNOS induction.
Takeaway
In mice with sepsis, the way white blood cells stick to blood vessels and how much fluid leaks out are controlled by different things, especially a substance called nitric oxide.
Methodology
Leukocyte dynamics and microvascular permeability were assessed using fluorescence intravital microscopy in mice made septic by cecal ligation and puncture.
Potential Biases
Potential confounding effects of mast cell degranulation and the effects of anesthesia cannot be ruled out.
Limitations
The study only tested responses in one vascular bed and did not assess microcirculatory flow and hematocrit.
Participant Demographics
C57Bl/6 mice, including wild-type and iNOS-deficient transgenic mice.
Statistical Information
P-Value
P < 0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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