Cell cycle times of short-term cultures of brain cancers as predictors of survival
2008

Culture Cycle Times of Brain Cancers and Survival

Sample size: 70 publication 10 minutes Evidence: high

Author Information

Author(s): Furneaux C E, Marshall E S, Yeoh K, Monteith S J, Mews P J, Sansur C A, Oskouian R J, Sharples K J, Baguley B C

Primary Institution: Auckland Hospital, Auckland, New Zealand

Hypothesis

Do culture cycle times of brain cancers correlate with patient survival?

Conclusion

Culture cycle times from short-term cultures of brain tumors are significantly related to patient survival.

Supporting Evidence

  • Patients with culture cycle times of ⩽10 days had a median survival of 5.1 months.
  • Patients with culture cycle times of >10 days had a median survival of 12.5 months.
  • Lower grade gliomas had longer median culture cycle times than glioblastomas and medulloblastomas.

Takeaway

This study found that how fast brain cancer cells grow in a lab can help predict how long patients might live.

Methodology

Brain tumor samples from patients were cultured for 7 days, and culture cycle times were estimated using 3H-thymidine incorporation.

Potential Biases

Potential bias from missing data on treatment and small sample sizes for certain tumor types.

Limitations

The study may be biased due to missing data on radiotherapy and the small sample size for some tumor types.

Participant Demographics

Patients included 41 males and 29 females with various brain tumors, including glioblastoma, medulloblastoma, and metastatic melanoma.

Statistical Information

P-Value

0.0009

Confidence Interval

95% CI (2.6–8.2) for ⩽10 days and 95% CI (9.0–18.4) for >10 days

Statistical Significance

p=0.0009

Digital Object Identifier (DOI)

10.1038/sj.bjc.6604716

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