MUC16 Expression in Sjogren’s Syndrome and Dry Eye Conditions
Author Information
Author(s): Caffery Barbara, Joyce Elizabeth, Heynen Miriam L., Jones Lyndon, Ritter Robert III, Gamache Daniel A., Senchyna Michelle
Primary Institution: University of Waterloo
Hypothesis
The study investigates the expression of MUC16 protein and mRNA in tears and conjunctival cells of Sjogren’s syndrome, keratoconjunctivitis sicca, and non-dry eyed subjects.
Conclusion
Sjogren’s syndrome subjects have significantly higher levels of soluble MUC16 and MUC16 mRNA compared to those with keratoconjunctivitis sicca and non-dry eyed individuals.
Supporting Evidence
- Sjogren’s syndrome subjects showed higher soluble MUC16 levels compared to KCS and NDE groups.
- No significant correlation was found between tear flow and MUC16 expression.
- Five of the 25 SS subjects did not provide sufficient tear samples for analysis.
Takeaway
People with Sjogren’s syndrome have more of a specific protein called MUC16 in their tears compared to those who don't have dry eyes.
Methodology
The study involved 76 subjects divided into three groups: Sjogren’s syndrome, keratoconjunctivitis sicca, and non-dry eyed controls, with tear flow measured and MUC16 quantified using western blotting and qPCR.
Potential Biases
Potential bias due to the small number of subjects and the specific recruitment methods used.
Limitations
The study had a small sample size and some subjects did not provide sufficient tear samples for analysis.
Participant Demographics
Mean age of SS group was 60 years, KCS group was 59 years, and NDE group was 52 years, with a majority of female subjects.
Statistical Information
P-Value
p=0.004
Statistical Significance
p<0.05
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