RsaI repetitive DNA in Buffalo Bubalus bubalis representing retrotransposons, conserved in bovids, are part of the functional genes
2011
Study of RsaI Repetitive DNA in Buffalo
publication
Evidence: moderate
Author Information
Author(s): Pathak Deepali, Ali Sher
Primary Institution: National Institute of Immunology
Hypothesis
RsaI repeats in the buffalo genome may play a role in the architecture and evolution of the genome.
Conclusion
RsaI repeats are integrated into functional genes and are transcriptionally active in buffalo somatic tissues and spermatozoa.
Supporting Evidence
- RsaI repeats were found to be transcriptionally active in various buffalo tissues.
- The study identified four RsaI repeat fragments with varying copy numbers across buffalo, cattle, goat, and sheep.
- Fluorescence in situ hybridization showed RsaI repeats distributed across buffalo chromosomes.
Takeaway
This study found that certain DNA sequences in buffalo are important for their genes and are active in their cells.
Methodology
The study involved cloning, sequencing, and analyzing RsaI repeat fragments from buffalo genomic DNA, along with Real Time PCR and fluorescence in situ hybridization.
Digital Object Identifier (DOI)
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