Analysis of FRT-Like Sequences in the Human Genome
Author Information
Author(s): Shultz Jeffry L., Voziyanova Eugenia, Konieczka Jay H., Voziyanov Yuri
Primary Institution: School of Biological Sciences, Louisiana Tech University
Hypothesis
To understand the distribution and sequence properties of genomic FRT-like sites in the human genome.
Conclusion
The study identified FRT-like sequences in the human genome roughly every 5 kb and classified them into unique and duplicated groups.
Supporting Evidence
- The analysis identified 642,151 potentially functional FRT-like sequences in the human genome.
- Out of those, 581,157 FRT-like sequences were unique.
- The study found that duplicated FRT-like sequences mainly originate from LINE1, LTRs, and Alu repeats.
- The identified FRT-like sequences were grouped into classes based on their sequence features.
Takeaway
Scientists looked at special DNA sequences in humans that are similar to a known sequence and found many of them, which can help in gene editing.
Methodology
A genome-wide analysis was performed using a bioinformatics package called TargetSiteAnalyzer to identify and classify FRT-like sequences.
Limitations
The study did not investigate the preference of FRT-like sequences to be in exons or introns per arbitrary unit of DNA length.
Digital Object Identifier (DOI)
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