Triage of Women with Low-Grade Cervical Lesions - HPV mRNA Testing versus Repeat Cytology
2011

HPV mRNA Testing vs. Repeat Cytology for Women with Low-Grade Cervical Lesions

Sample size: 522 publication Evidence: high

Author Information

Author(s): Sørbye Sveinung Wergeland, Arbyn Marc, Fismen Silje, Gutteberg Tore Jarl, Mortensen Elin Synnøve

Primary Institution: University Hospital of North Norway

Hypothesis

Is HPV mRNA testing more effective than repeat cytology in triaging women with low-grade squamous intraepithelial lesions (LSIL)?

Conclusion

HPV mRNA testing was found to be more sensitive and specific than repeat cytology for triaging women with LSIL.

Supporting Evidence

  • HPV mRNA testing showed a sensitivity of 94.2% and specificity of 86.0%.
  • The positive predictive value (PPV) of HPV mRNA testing was 67.0%, compared to 38.4% for repeat cytology.
  • 125 out of 522 women (23.9%) had CIN2+ confirmed by biopsy.

Takeaway

This study shows that a test for HPV mRNA is better than a Pap smear for checking if women with mild cervical changes need more tests.

Methodology

Women with LSIL were divided into two groups: one received repeat cytology only, and the other received both repeat cytology and HPV mRNA testing.

Potential Biases

Potential bias due to the influence of HPV results on cytological diagnosis.

Limitations

The study lacked formal randomization, which may have led to imbalanced groups.

Participant Demographics

Women aged 25-69 years with low-grade squamous intraepithelial lesions.

Statistical Information

P-Value

p<0.001

Confidence Interval

95% CI: 72.1, 92.2 for sensitivity of repeat cytology; 95% CI: 88.7, 99.7 for sensitivity of HPV mRNA test.

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024083

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication