Differentiating Enterobacter sakazakii Genotypes
Author Information
Author(s): Carol Iversen, Mike Waddington, Jim J Farmer III, Stephen J Forsythe
Primary Institution: Nottingham Trent University
Hypothesis
This study aims to compare the genotypes of Enterobacter sakazakii strains with their biochemical traits.
Conclusion
The study found a strong agreement between genotyping and biotyping of Enterobacter sakazakii and identified a new biogroup.
Supporting Evidence
- The study analyzed 189 strains of E. sakazakii.
- Four distinct DNA cluster groups were identified.
- A new biogroup (Biogroup 16) was defined.
- The majority of strains clustered with the type strain.
Takeaway
Scientists looked at different types of a germ called Enterobacter sakazakii and found new ways to tell them apart.
Methodology
The study analyzed 189 strains of E. sakazakii using partial 16S rDNA sequencing and biochemical tests.
Limitations
The study did not use DNA-DNA hybridization, which is the standard for species delineation.
Participant Demographics
Strains were from diverse food, clinical, and environmental sources worldwide.
Digital Object Identifier (DOI)
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