IgE Immune Complexes Increase Lung Mast Cell Progenitors in Mice
Author Information
Author(s): Dahlin Joakim S., Ivarsson Martin A., Heyman Birgitta, Hallgren Jenny
Primary Institution: Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
Hypothesis
Formation of IgE immune complexes in the lungs of sensitized mice increases the migration of mast cell progenitors to this organ.
Conclusion
IgE immune complex challenge enhances the number of lung mast cell progenitors through activation of an Fc receptor associated with the FcRγ chain.
Supporting Evidence
- IgE immune complex challenge elicited three times more mast cell progenitors per lung than challenge with the same dose of antigen alone.
- The enhancement of lung mast cell progenitors by IgE immune complex challenge was lost in FcRγ deficient mice.
- IgE immune complex challenge increased the frequency of mast cell progenitors per 106 mononuclear cells by 2.1-fold.
Takeaway
When mice with allergies are given a special type of immune complex, it helps more baby mast cells move into their lungs, which can make allergies worse.
Methodology
Mice were sensitized with ovalbumin and challenged with either OVA-TNP alone or IgE-anti-TNP/OVA-TNP immune complexes, followed by quantification of mast cell progenitors.
Limitations
The study was limited by the inability to perform individual experiments with enough animals for statistical analysis.
Participant Demographics
6–14 week old male BALB/c, CD23−/−, and FcRγ−/− mice were used.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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