Inhibition of PP2A by LIS1 increases HIV-1 gene expression
2006
LIS1 Increases HIV-1 Gene Expression
Sample size: 293
publication
Evidence: moderate
Author Information
Author(s): Epie Nicolas, Ammosova Tatyana, Turner Willie, Nekhai Sergei
Primary Institution: Howard University College of Medicine
Hypothesis
Does LIS1 enhance HIV-1 transcription by inhibiting PP2A?
Conclusion
Inhibition of PP2A by LIS1 induces HIV-1 transcription.
Supporting Evidence
- LIS1 was shown to increase Tat-mediated HIV-1 transcription in 293 cells.
- The WD5 domain of LIS1 was identified as crucial for enhancing HIV-1 transcription.
- LIS1 inhibits the activity of protein phosphatase 2A (PP2A), which is linked to HIV-1 transcription regulation.
Takeaway
LIS1 helps a virus called HIV-1 make more copies of itself by stopping a protein that usually slows it down.
Methodology
The study involved transfecting 293 cells with LIS1 expression vectors and measuring HIV-1 transcription levels.
Participant Demographics
293 cells, a human cell line.
Digital Object Identifier (DOI)
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