Characterization of the minimum domain required for targeting budding yeast myosin II to the site of cell division
2006
Understanding How Yeast Myosin II Localizes During Cell Division
publication
Evidence: moderate
Author Information
Author(s): Ida Lister, Nicola J Tolliday, Rong Li
Primary Institution: Harvard Medical School
Hypothesis
What is the minimum domain required for targeting budding yeast myosin II to the site of cell division?
Conclusion
The study identifies a small region in the tail of budding yeast myosin II that is necessary and sufficient for its localization to the site of cell division.
Supporting Evidence
- The minimum localization domain (MLD) of Myo1 is necessary for its localization to the yeast bud neck.
- Hydrodynamic measurements suggest that the MLD forms a trimer.
- Deletion of the hinge region within the MLD affects the contraction of the contractile ring.
Takeaway
Scientists found a tiny part of a protein that helps yeast cells know where to divide. This part is really important for the cell to work properly.
Methodology
The study used molecular genetics, biochemistry, and live cell imaging to analyze the minimum localization domain of budding yeast Myo1.
Digital Object Identifier (DOI)
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