Mouse Oocytes Fertilized During Maturation Can Be Activated After Refertilization
Author Information
Author(s): Jędrusik Agnieszka, Ajduk Anna, Pomorski Paweł, Maleszewski Marek
Primary Institution: Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
Hypothesis
Does the premature introduction of spermatozoa into maturing oocytes affect their ability to be activated and generate calcium responses after maturation?
Conclusion
Mouse oocytes fertilized during maturation can still be activated and generate calcium oscillations after refertilization.
Supporting Evidence
- Refertilised oocytes activated with a frequency similar to control oocytes.
- Refertilised oocytes generated fewer calcium transients than control oocytes.
- IP3 receptor levels were lower in oocytes fertilised during maturation.
Takeaway
When mouse eggs are fertilized too early, they can still wake up and respond to another fertilization later on.
Methodology
Mouse oocytes were fertilized by ICSI during maturation and then refertilized at metaphase II to assess activation and calcium responses.
Potential Biases
Potential bias in the selection of oocytes and the experimental conditions.
Limitations
The study primarily focuses on mouse oocytes, which may not fully represent other species.
Participant Demographics
F1 (C57Bl/6 × CBA/H and CBA/H × C57Bl/6) female and male mice were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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