Hypomethylation and Expression of MN/CA9 in Kidney Cancer
Author Information
Author(s): Cho M, Uemura H, Kim S-C, Kawada Y, Yoshida K, Hirao Y, Konishi N, Saga S, Yoshikawa K
Primary Institution: Nara Medical University
Hypothesis
The study investigates the relationship between the methylation status of the MN/CA9 promoter region and its expression in human renal cell carcinomas.
Conclusion
Hypomethylation of a specific CpG site in the MN/CA9 promoter is linked to increased expression of the MN/CA9 gene in renal cell carcinoma.
Supporting Evidence
- MN/CA9 expression was observed in 10 of 13 RCCs (77%).
- 9 out of 10 MN/CA9-positive RCCs (90%) contained clear cell components.
- 69% of RCCs showed partial hypomethylation of the CpG at −74 bp.
- Hypomethylation of the CpG at −74 bp was strongly correlated with MN/CA9 expression.
- Methylation of the CpG at −74 bp strongly suppressed MN/CA9 promoter activity.
Takeaway
In kidney cancer, a part of the gene that helps it grow is often not turned off because it has lost a chemical tag, which helps it stay active.
Methodology
The study used RT-PCR to evaluate MN/CA9 expression and bisulfite genomic sequencing to assess methylation status in human RCCs.
Digital Object Identifier (DOI)
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