Proteins interacting with Caenorhabditis elegans G subunits
2003
Proteins Interacting with C. elegans G Subunits
Sample size: 24
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Edwin Cuppen, Alexander M. van der Linden, Gert Jansen, Ronald H. A. Plasterk
Primary Institution: Hubrecht Laboratory, Utrecht, The Netherlands
Hypothesis
To identify novel components in heterotrimeric G-protein signalling.
Conclusion
The study identified 11 different proteins that interact with four different G subunits in C. elegans.
Supporting Evidence
- The study used a large-scale yeast two-hybrid experiment to identify protein interactions.
- 24 clones representing 11 different proteins were identified as interacting with G subunits.
- Expression patterns of interacting proteins were analyzed using promoter-GFP reporter constructs.
Takeaway
The researchers looked for new proteins that work with G-proteins in tiny worms and found 11 that do.
Methodology
Yeast two-hybrid interaction screens were performed to identify proteins interacting with G subunits.
Limitations
The study may not have identified all interacting proteins due to potential issues with protein folding and expression levels.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website