Rapid Redistribution of Synaptic PSD-95 in the Neocortex In Vivo
2006

How PSD-95 Affects Neuronal Connections

publication Evidence: moderate

Author Information

Author(s): Karel Svoboda, Rachel Jones

Hypothesis

How does the protein PSD-95 spread within cells and modulate the strength and size of neuronal connections?

Conclusion

The study found that the turnover of PSD-95 is crucial for maintaining the appropriate size of the postsynaptic density at each synapse.

Supporting Evidence

  • PSD-95 clusters were stable for days in some instances.
  • Large spines contain more PSD-95 and are more stable than smaller ones.
  • PSD-95 retention increases with age during development.

Takeaway

This study shows that a protein called PSD-95 helps keep connections between brain cells strong and stable, and it can move around to help with that.

Methodology

The authors used dual-laser two-photon laser scanning microscopy to observe PSD-95 in living neurons of embryonic mice.

Participant Demographics

Embryonic mice were used in the study.

Digital Object Identifier (DOI)

10.1371/journal.pbio.0040404

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