CBR3 and Retinoic Acid in Oral Cancer
Author Information
Author(s): Ohkura-Hada Shuri, Kondoh Nobuo, Hada Akiyuki, Arai Masaaki, Yamazaki Yutaka, Shindoh Masanobu, Kitagawa Yoshimasa, Takahashi Masayuki, Ando Toshifumi, Sato Yasunori, Yamamoto Mikio
Primary Institution: National Defense Medical College
Hypothesis
The cytostatic effects of retinoic acid could be mediated by the activation of the CBR3 gene in oral squamous cell carcinomas.
Conclusion
The expression of CBR3 is significantly reduced in highly invasive oral squamous cell carcinomas compared to pre-malignant lesions.
Supporting Evidence
- 9-cis-retinoic acid was shown to suppress growth in OSCC cells.
- CBR3 expression was significantly higher in pre-cancerous tissues compared to OSCCs.
- The study identified 14 up-regulated and 17 down-regulated genes in RA-treated cells.
Takeaway
This study found that a gene called CBR3 helps stop cancer cells from growing when treated with a substance called retinoic acid, and lower levels of this gene are linked to more aggressive cancers.
Methodology
The study used human OSCC cell lines and tissue samples, performing cDNA microarray analysis and quantitative RT-PCR to assess gene expression.
Limitations
The study did not assess the invasive potential of CBR3-transfected cells in vivo due to the lack of tumorigenicity in the cell line used.
Participant Demographics
The study included 64 OSCC samples and 19 leukoplakia tissues.
Statistical Information
P-Value
p<0.018
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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