Efficient Isolation and Identification of Human Adipose-Derived Stem Cells
Author Information
Author(s): Yang Xu-Fang, He Xu, He Jian, Zhang Li-Hong, Su Xue-Jin, Dong Zhi-Yong, Xu Yun-Jian, Li Yan, Li Yu-Lin
Primary Institution: Jilin University
Hypothesis
The study aims to develop high efficient methods to isolate and identify human adipose-derived mesenchymal stem cells (hADSCs).
Conclusion
The study developed efficient methods for isolating and cultivating hADSCs, providing a systematic strategy for their identification and characterization.
Supporting Evidence
- The yield of hADSCs reached around 1 × 10^6 per gram of adipose tissue.
- hADSCs exhibited typical fibroblast-like morphology and normal karyotype.
- The cells expressed high levels of MSC-specific markers and showed no contamination from hematopoietic lineage.
Takeaway
Scientists found a better way to get stem cells from fat tissue, which can help in medical research and treatments.
Methodology
hADSCs were isolated from abdominal adipose tissue using collagenase digestion and adherence to flasks.
Participant Demographics
Healthy obese women aged 23-41 years.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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