Best Reference Genes for Breast Cancer Biomarker Quantification
Author Information
Author(s): Majidzadeh-A Keivan, Esmaeili Rezvan, Abdoli Nasrin
Primary Institution: Iranian Center for Breast Cancer (ICBC); Academic Center for Education, Culture and Research (ACECR)
Hypothesis
The study aims to identify uniformly expressed housekeeping genes for breast cancer gene expression studies.
Conclusion
TFRC and ACTB are the best combination of genes for quantifying Urokinase Plasminogen Activator in breast cancer.
Supporting Evidence
- TFRC was identified as the most stable housekeeping gene.
- RPLP0 and GAPDH were found to be the least stable genes.
- GeNorm and NormFinder software were used to analyze gene stability.
Takeaway
The researchers found that two specific genes are the best to use when measuring a cancer-related protein in breast cancer samples.
Methodology
The study analyzed the expression of six housekeeping genes in breast tumor, normal, and adjacent tissues using quantitative Real-Time PCR.
Limitations
The study suggests testing with a larger sample size and more candidate reference genes to find more stable endogenous controls.
Participant Demographics
Participants included 40 breast tumor samples, 4 normal tissues, and 8 adjacent tissues from Iranian women.
Statistical Information
P-Value
<0.05
Confidence Interval
95%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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