A comparison of the expression of the DIDS-binding proteins from normal and tumorigenic human cells
1984
Comparison of DIDS-Binding Proteins in Normal and Tumorigenic Human Cells
publication
Evidence: moderate
Author Information
Author(s): M.R.C. Banyard
Primary Institution: John Curtin School of Medical Research, Australian National University
Hypothesis
How do DIDS-binding proteins differ between tumorigenic and non-tumorigenic human cells?
Conclusion
The study found no difference in the number of DIDS-binding sites between tumorigenic and non-tumorigenic cells.
Supporting Evidence
- The DIDS-binding protein occurs homogeneously on all cells.
- HEp-2 cells had significantly more DIDS-binding sites than MRC-5 cells.
- The binding of DIDS does not compete with M/27 but enhances its binding.
Takeaway
The researchers looked at how a specific protein that binds to a chemical called DIDS behaves in cancerous and normal cells, and they found that both types of cells have the same amount of this protein.
Methodology
Monoclonal antibodies were used to compare DIDS-binding sites in various human cell types.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
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