Deep Two-Photon Voltage Imaging with Adaptive Excitation
Author Information
Author(s): Zhao Shitong, Hebert Eric, Gruzdeva Anna, Mahishi Deepthi, Takahashi Hazuki, Lee Sungmoo, Hao Yukun A., Lin Michael Z., Yapici Nilay, Xu Chris
Primary Institution: Cornell University
Hypothesis
Can adaptive excitation improve deep voltage imaging in awake mouse brains?
Conclusion
The study demonstrates a new method for high-speed, deep voltage imaging of multiple neurons in the mouse brain using adaptive excitation.
Supporting Evidence
- The adaptive excitation method allows for imaging at depths of 500-630 μm in the mouse brain.
- Using adaptive excitation, the study achieved a 40- to 50-fold increase in photon collection per neuron.
- The method enables simultaneous imaging of multiple neurons across two planes.
- Fluorescence traces showed a significant improvement in signal-to-noise ratio with adaptive excitation.
Takeaway
This study shows a new way to take pictures of brain activity in mice without hurting them, using special light techniques to see deep inside their brains.
Methodology
The study used a two-photon microscope with an adaptive excitation source to image neuronal voltage activities in awake mice.
Limitations
The imaging depth is limited to 700 μm, and the method may not be applicable to all types of voltage indicators.
Participant Demographics
C57BL/6J mice, both male and female, aged 2-6 months.
Digital Object Identifier (DOI)
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