Measuring Apoptosis in Breast Tissue
Author Information
Author(s): Hadjiloucas I, Gilmore A P, Bundred N J, Streuli C H
Primary Institution: University of Manchester
Hypothesis
The study investigates the relationship between apoptosis measured by caspase 3 activation and tumor histopathological characteristics in breast tissue.
Conclusion
The study found that measuring apoptosis using caspase 3 activation is reliable and correlates well with traditional morphological assessments.
Supporting Evidence
- The apoptotic index obtained by measuring caspase activation correlates strongly with the index derived from morphological assessment.
- The apoptotic index is higher in invasive breast cancer compared to benign cases.
- In invasive cancer, a high apoptotic index is associated with high tumor grade and positive node status.
Takeaway
The researchers looked at breast tissue samples to see how many cells were dying and found a good way to measure this using a special test.
Methodology
Immunohistochemistry was used to measure the apoptotic index in breast tissue samples.
Participant Demographics
22 normal/benign and 94 invasive carcinoma samples.
Statistical Information
P-Value
P = 0.008
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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