Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection
2008

PCR is Better than Serology for Diagnosing Mycoplasma pneumoniae Infection

Sample size: 164 publication Evidence: high

Author Information

Author(s): Nilsson Anna C, Björkman Per, Persson Kenneth

Primary Institution: Lund University, Malmö University Hospital, Sweden

Hypothesis

Is PCR more effective than serology for diagnosing Mycoplasma pneumoniae infections during the early stages of illness?

Conclusion

PCR is superior to serology for diagnosing Mycoplasma pneumoniae infection during the early phases of infection.

Supporting Evidence

  • PCR detected 93% of patients who later developed an antibody response to Mycoplasma pneumoniae.
  • Only 21% of patients had a diagnostic antibody reaction in their acute phase serum during the first week of illness.
  • The median time for carriage of Mycoplasma pneumoniae DNA was 7 weeks after disease onset.

Takeaway

Doctors can find out if someone has a Mycoplasma pneumoniae infection faster with a special test called PCR instead of waiting for blood tests.

Methodology

The study compared PCR and serology for diagnosing Mycoplasma pneumoniae infection in patients with respiratory symptoms during an outbreak.

Potential Biases

Patients with more severe symptoms may have been overrepresented due to recruitment methods.

Limitations

The study mainly included patients from a hospital, which may not represent the general population and could overestimate the severity of cases.

Participant Demographics

164 patients (62% women, 38% men; median age 41 years, range 2-82).

Statistical Information

P-Value

0.16

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2180-8-93

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