Study of Clade-1 Vap Proteins in Rhodococcus equi
Author Information
Author(s): Zeynep Yerlikaya, Raúl Miranda-CasoLuengo, Yuting Yin, Cheng Cheng, Wim G. Meijer
Primary Institution: University College Dublin
Hypothesis
Cell surface localization is an important characteristic of clade-1 Vap proteins that support intracellular growth.
Conclusion
Only clade-1 Vap proteins are necessary for intracellular growth in macrophages and are the only ones associated with the R. equi cell surface.
Supporting Evidence
- Only clade-1 Vap proteins support intracellular growth in macrophages.
- VapA is the only protein located on the cell surface among the six equine Vap proteins.
- VapK1 and VapK2 are functionally homologous to VapA and also support growth.
Takeaway
Some proteins help a germ called Rhodococcus equi grow inside certain cells, and only a specific group of these proteins can stick to the outside of the germ.
Methodology
The study involved examining the ability of 17 Vap proteins to support intracellular growth in a R. equi ΔvapA strain using various complementation assays.
Limitations
The study does not explore the functions of Vap proteins from other clades.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website