Protecting Insulin from Degradation in the Intestine
Author Information
Author(s): Zhang Liefeng, Jiang Hui, Zhu Wenjie, Wu Lin, Wu Lingling, Ren Qiuyan, Yong Ren
Primary Institution: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University
Hypothesis
Can casein, protamine, and HP-β-CD protect insulin from degradation by intestinal enzymes?
Conclusion
Casein and protamine can help protect insulin from degradation in the intestine, especially when combined with HP-β-CD.
Supporting Evidence
- Insulin was rapidly degraded by α-chymotrypsin, but casein and protamine significantly protected it.
- Co-incubation with HP-β-CD and casein resulted in about 80% of insulin remaining undegraded.
- HP-β-CD alone had little effect on insulin stability, but casein showed significant protective effects.
Takeaway
This study found that certain proteins and compounds can help keep insulin safe in the stomach so it can work better when taken by mouth.
Methodology
Insulin degradation was studied using HPLC and PAGE methods to measure remaining insulin after treatment with intestinal enzymes.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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