E2F8 is a nonreceptor activator of heterotrimeric G proteins
2007
E2F8 Activates G Proteins
publication
Evidence: moderate
Author Information
Author(s): Ian S. Hagemann, Kirk D. Narzinski, Thomas J. Baranski
Primary Institution: Washington University in St. Louis
Hypothesis
Can E2F8 act as a nonreceptor activator of heterotrimeric G proteins?
Conclusion
E2F8 is a guanine nucleotide exchange factor for the α subunit of heterotrimeric G proteins in yeast.
Supporting Evidence
- E2F8 was identified through a yeast expression cloning strategy.
- E2F8 specifically activated Gαi over Gpa1.
- The entire E2F8 protein was required for full activity.
Takeaway
E2F8 helps cells send signals by turning on special proteins called G proteins, even without a receptor.
Methodology
Yeast expression cloning was used to identify E2F8's role in activating G proteins.
Limitations
The physiological significance of E2F8's activity in mammalian cells remains unclear.
Statistical Information
P-Value
p<0.05
Confidence Interval
95% CI, 120–170 nM
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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